Associate Professor
15 years of experience
I am a molecular plant breeding specialist with extensive experience operating, maintaining, troubleshooting, and training users on advanced scientific equipment. I have worked hands-on with thermal cyclers and real-time PCR systems (e.g., Applied Biosystems QuantStudio, Bio-Rad CFX96), automated DNA/RNA extraction platforms (Qiagen QIAcube, Thermo Fisher KingFisher Flex), Illumina MiSeq and HiSeq sequencers, and Sanger sequencing instruments (ABI 3500 Genetic Analyzer). My expertise includes installing and calibrating these systems, performing routine maintenance, resolving hardware and software issues, and ensuring compliance with Good Laboratory Practice standards. I have led training workshops, developed SOPs and technical manuals, and guided research teams in high-throughput genotyping and sequencing workflows. My background includes supporting marker-assisted selection pipelines, optimizing molecular assays, and collaborating with vendors and service engineers on instrument setup and validation. This combination of technical proficiency, project management, and capacity building has contributed to efficient and reliable molecular breeding and genomics operations.
In my specialization in molecular plant breeding, I work extensively with advanced laboratory equipment critical for high-resolution genotyping, molecular marker development, and functional genomics. This includes thermal cyclers and real-time PCR platforms such as the Applied Biosystems QuantStudio and Bio-Rad CFX96 systems for quantitative gene expression analysis and SNP genotyping, as well as automated DNA and RNA extraction platforms like the Qiagen QIAcube and Thermo Fisher KingFisher Flex to ensure consistent nucleic acid quality. For sequencing applications, I am proficient in operating next-generation sequencers, notably the Illumina MiSeq and HiSeq systems, along with Sanger sequencing workflows using ABI 3500 Genetic Analyzers. My work also involves the use of high-throughput genotyping platforms, such as KASP and Kompetitive Allele Specific PCR systems, and fragment analysis on capillary electrophoresis instruments. Additionally, I routinely employ gel electrophoresis units, NanoDrop spectrophotometers, Qubit fluorometers, and automated liquid handling workstations to streamline sample preparation and quantification. In plant tissue culture and transformation, I utilize laminar flow cabinets, growth chambers with precise environmental controls, and bioreactors for in vitro propagation and regeneration of transgenic lines. Collectively, these technologies underpin the integration of molecular and genomic tools into modern breeding pipelines and enable the efficient development of improved crop varieties.
https://scholar.google.com/citations?user=9x9EzdsAAAAJ&hl=en
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