Professor, Department of Forensic Medicine University of Health Sciences, Lahore
15 years of experience
I am an experienced forensic genetics professional with a strong background in the operation, maintenance, troubleshooting, and user training of specialized molecular biology and DNA analysis equipment used in forensic, medical, and research laboratories. My expertise lies in systems central to human identification and DNA profiling, including capillary electrophoresis platforms, next-generation sequencing (NGS) systems, automated DNA extraction instruments, real-time PCR quantification systems, and DNA database management tools.
Over the years, I have developed deep hands-on experience with Applied Biosystems 3500/3130xl Genetic Analyzers, used for STR profiling and fragment analysis. I routinely conduct preventive maintenance tasks such as capillary array replacements, polymer refills, and spectral calibration, ensuring optimal instrument performance and minimal downtime. I also assist users in troubleshooting injection failures, peak quality issues, and software configuration errors using GeneMapper ID-X.
In the field of NGS, I have worked extensively with platforms such as the Illumina MiSeq FGx and Ion Torrent systems, supporting forensic applications including SNP analysis, mitochondrial DNA sequencing, and ancestry inference. My responsibilities have included instrument care, reagent and flow cell handling, troubleshooting run failures, and providing detailed training on library preparation workflows. I am also experienced with associated software tools such as ForenSeq Universal Analysis Software and Torrent Suite, guiding users through data quality checks, alignment, and variant interpretation.
I am proficient with automated extraction platforms like the Qiagen QIAcube, Promega Maxwell RSC, and real-time PCR instruments like the QuantStudio 5 for DNA quantification. I ensure these systems remain within calibration, address common mechanical issues (e.g., robotic arm errors or pipetting inconsistencies), and train lab personnel on SOP-compliant usage. I also provide guidance on the use of commercial forensic kits, including GlobalFiler, PowerPlex, and Quantifiler, supporting both routine casework and research validation projects.
My role extends to the management and operation of forensic DNA databases (e.g., CODIS-compatible systems) where I assist in the validation of DNA profiles, interpretation of allelic drop-out/drop-in events, and database entry procedures. I also provide training on maintaining chain-of-custody integrity and data security protocols within LIMS environments.
Throughout my career, I have led several training workshops for new analysts, interns, and academic researchers. I have developed comprehensive user manuals and quick-reference SOPs tailored to specific instruments, covering maintenance checklists, troubleshooting guides, and best-practice workflows. These materials have improved lab efficiency, reduced errors, and empowered users to operate equipment independently.
Additionally, I stay current with advances in forensic technology through vendor training programs, webinars, and certification courses. I have completed factory training on capillary electrophoresis maintenance and received vendor-led instruction on MiSeq FGx operation and NGS library troubleshooting.
Overall, my combined technical and instructional experience allows me to ensure that advanced forensic equipment is not only well-maintained and functional but also optimally utilized by all members of the lab.
I specialize in advanced equipment and technologies used in forensic genetics, with a strong focus on DNA profiling, molecular analysis, and next-generation sequencing (NGS). My expertise includes:
Capillary Electrophoresis Systems (e.g., Applied Biosystems 3500/3130xl Genetic Analyzers): Extensive experience in short tandem repeat (STR) analysis, DNA fragment separation, and genotyping for forensic casework and databasing.
Next-Generation Sequencing Platforms (e.g., Illumina MiSeq FGx, Thermo Fisher Ion Torrent): Skilled in library preparation, quality control, data generation, and analysis for massively parallel sequencing applications in forensic and ancestry testing.
DNA Extraction and Quantification Systems (e.g., QIAcube, Maxwell RSC, QuantStudio, Quantifiler kits): Proficient in automated and manual extraction methods, as well as accurate quantification of degraded or trace samples.
PCR Amplification Systems (e.g., Veriti Thermal Cycler, GeneAmp PCR Systems): Hands-on experience with forensic STR kits (e.g., GlobalFiler, PowerPlex) for multiplex amplification and DNA profiling.
Forensic DNA Databases (e.g., CODIS, STRmix-compatible databases): Familiar with database management, entry validation, and interpretation of DNA profiles within national and international forensic DNA databasing systems.
Bioinformatics and Data Interpretation Tools: Skilled in using software for sequence alignment, variant calling, and STR interpretation (e.g., GeneMapper, ForenSeq Universal Analysis Software, OSIRIS).
Quality Assurance and Chain of Custody Systems: Experience with forensic laboratory information management systems (LIMS), audit compliance, and documentation protocols in accredited forensic labs (e.g., ISO/IEC 17025 environments).
These systems and platforms are integral to forensic casework, missing persons identification, disaster victim identification (DVI), and research applications. My role typically involves hands-on operation, troubleshooting, data interpretation, and training of forensic practitioners and students on best practices and analytical workflows in forensic DNA analysis.
https://www.uhs.edu.pk/for-staff.php https://www.researchgate.net/profile/Allah-Rakha-3
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